Construct: shRNA TRCN0000426758

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011695.2-1302s21c1
DNA Barcode:
GCCATGGTGTGCATGTTTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vdac2 (22334)
Gene Description:
voltage-dependent anion channel 2
Transcript:
RefSeq NM_011695.2 (CURRENT)
Match location:
Position 1302 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426758)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGGTGTGCATGTTTGTT
Hairpin Sequence:
5'-CCGG-GCCATGGTGTGCATGTTTGTT-CTCGAG-AACAAACATGCACACCATGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGGTGTGCATGTTTGTTCTCGAGAACAAACATGCACACCATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGGTGTGCATGTTTGTTCTCGAGAACAAACATGCACACCATGGC-3'

Other clones with same target sequence:

(none)