Construct: shRNA TRCN0000426847

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177360.3-1813s21c1
DNA Barcode:
GACTGTGCTTGATAGTCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dmrt3 (240590)
Gene Description:
doublesex and mab-3 related transcription factor 3
Transcript:
RefSeq NM_177360.3 (CURRENT)
Match location:
Position 1813 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426847)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTGTGCTTGATAGTCTATA
Hairpin Sequence:
5'-CCGG-GACTGTGCTTGATAGTCTATA-CTCGAG-TATAGACTATCAAGCACAGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTGTGCTTGATAGTCTATACTCGAGTATAGACTATCAAGCACAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTGTGCTTGATAGTCTATACTCGAGTATAGACTATCAAGCACAGTC-3'

Other clones with same target sequence:

(none)