Construct: shRNA TRCN0000426867

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145833.1-995s21c1
DNA Barcode:
TAGAAGGCTGTGTGATATTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lin28a (83557)
Gene Description:
lin-28 homolog A (C. elegans)
Transcript:
RefSeq NM_145833.1 (CURRENT)
Match location:
Position 995 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000426867)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGAAGGCTGTGTGATATTTC
Hairpin Sequence:
5'-CCGG-TAGAAGGCTGTGTGATATTTC-CTCGAG-GAAATATCACACAGCCTTCTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGAAGGCTGTGTGATATTTCCTCGAGGAAATATCACACAGCCTTCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGAAGGCTGTGTGATATTTCCTCGAGGAAATATCACACAGCCTTCTA-3'

Other clones with same target sequence:

(none)