Construct: shRNA TRCN0000427383

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201354.2-795s21c1
DNA Barcode:
GTATCAGCCTGAATGACATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctif (269037)
Gene Description:
CBP80/20-dependent translation initiation factor
Transcript:
RefSeq NM_201354.2 (CURRENT)
Match location:
Position 795 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427383)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATCAGCCTGAATGACATTG
Hairpin Sequence:
5'-CCGG-GTATCAGCCTGAATGACATTG-CTCGAG-CAATGTCATTCAGGCTGATAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATCAGCCTGAATGACATTGCTCGAGCAATGTCATTCAGGCTGATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATCAGCCTGAATGACATTGCTCGAGCAATGTCATTCAGGCTGATAC-3'

Other clones with same target sequence:

(none)