Construct: shRNA TRCN0000427444
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_175543.3-2360s21c1
- DNA Barcode:
- CTGTTACATTCTCCAGAATTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Rab11fip4 (268451)
- Gene Description:
- RAB11 family interacting protein 4 (class II)
- Transcript:
- RefSeq NM_175543.3 (CURRENT)
- Match location:
- Position 2360 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000427444)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTGTTACATTCTCCAGAATTT
- Hairpin Sequence:
-
5'-CCGG-CTGTTACATTCTCCAGAATTT-CTCGAG-AAATTCTGGAGAATGTAACAG-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTGTTACATTCTCCAGAATTTCTCGAGAAATTCTGGAGAATGTAACAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTGTTACATTCTCCAGAATTTCTCGAGAAATTCTGGAGAATGTAACAG-3'
Other clones with same target sequence:
(none)
