Construct: shRNA TRCN0000427621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001002920.1-472s21c1
DNA Barcode:
GGAGGATGAATGTGCGTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POTEA (340441)
Gene Description:
POTE ankyrin domain family member A
Transcript:
RefSeq NM_001002920.1 (CURRENT)
Match location:
Position 472 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAGGATGAATGTGCGTTAAT
Hairpin Sequence:
5'-CCGG-GGAGGATGAATGTGCGTTAAT-CTCGAG-ATTAACGCACATTCATCCTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAGGATGAATGTGCGTTAATCTCGAGATTAACGCACATTCATCCTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAGGATGAATGTGCGTTAATCTCGAGATTAACGCACATTCATCCTCC-3'

Other clones with same target sequence:

(none)