Construct: shRNA TRCN0000427685

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145501.2-1934s21c1
DNA Barcode:
GCCCTTAATCTAGAACCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pi4k2a (84095)
Gene Description:
phosphatidylinositol 4-kinase type 2 alpha
Transcript:
RefSeq NM_145501.2 (CURRENT)
Match location:
Position 1934 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427685)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTAATCTAGAACCTAAA
Hairpin Sequence:
5'-CCGG-GCCCTTAATCTAGAACCTAAA-CTCGAG-TTTAGGTTCTAGATTAAGGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTAATCTAGAACCTAAACTCGAGTTTAGGTTCTAGATTAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTAATCTAGAACCTAAACTCGAGTTTAGGTTCTAGATTAAGGGC-3'

Other clones with same target sequence:

(none)