Construct: shRNA TRCN0000427756

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026100.3-599s21c1
DNA Barcode:
ATTAGTGATTCCGAGATATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tctex1d1 (67344)
Gene Description:
Tctex1 domain containing 1
Transcript:
RefSeq NM_026100.3 (CURRENT)
Match location:
Position 599 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427756)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTAGTGATTCCGAGATATAA
Hairpin Sequence:
5'-CCGG-ATTAGTGATTCCGAGATATAA-CTCGAG-TTATATCTCGGAATCACTAAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTAGTGATTCCGAGATATAACTCGAGTTATATCTCGGAATCACTAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTAGTGATTCCGAGATATAACTCGAGTTATATCTCGGAATCACTAAT-3'

Other clones with same target sequence:

(none)