Construct: shRNA TRCN0000427941

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145963.2-1769s21c1
DNA Barcode:
GGTTGAACATCAGTGAGATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnj14 (211480)
Gene Description:
potassium inwardly-rectifying channel, subfamily J, member 14
Transcript:
RefSeq NM_145963.2 (CURRENT)
Match location:
Position 1769 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427941)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTTGAACATCAGTGAGATAC
Hairpin Sequence:
5'-CCGG-GGTTGAACATCAGTGAGATAC-CTCGAG-GTATCTCACTGATGTTCAACC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTTGAACATCAGTGAGATACCTCGAGGTATCTCACTGATGTTCAACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTTGAACATCAGTGAGATACCTCGAGGTATCTCACTGATGTTCAACC-3'

Other clones with same target sequence:

(none)