Construct: shRNA TRCN0000428294

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026047.4-2041s21c1
DNA Barcode:
AGGTTTGGATCGTCCTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf219 (72486)
Gene Description:
ring finger protein 219
Transcript:
RefSeq NM_026047.4 (CURRENT)
Match location:
Position 2041 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428294)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGTTTGGATCGTCCTTATTT
Hairpin Sequence:
5'-CCGG-AGGTTTGGATCGTCCTTATTT-CTCGAG-AAATAAGGACGATCCAAACCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGTTTGGATCGTCCTTATTTCTCGAGAAATAAGGACGATCCAAACCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGTTTGGATCGTCCTTATTTCTCGAGAAATAAGGACGATCCAAACCT-3'

Other clones with same target sequence:

(none)