Construct: shRNA TRCN0000428565

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144618.2-906s21c1
DNA Barcode:
TTAACCTCGCAAGCCTTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GABPB2 (126626)
Gene Description:
GA binding protein transcription factor subunit beta 2
Transcript:
RefSeq NM_144618.2 (NON-CURRENT)
Match location:
Position 906 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428565)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTAACCTCGCAAGCCTTATTT
Hairpin Sequence:
5'-CCGG-TTAACCTCGCAAGCCTTATTT-CTCGAG-AAATAAGGCTTGCGAGGTTAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTAACCTCGCAAGCCTTATTTCTCGAGAAATAAGGCTTGCGAGGTTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTAACCTCGCAAGCCTTATTTCTCGAGAAATAAGGCTTGCGAGGTTAA-3'

Other clones with same target sequence:

(none)