Construct: shRNA TRCN0000428749

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053259.2-300s21c1
DNA Barcode:
AGCCTAAGAATGTACAGTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prss28 (114661)
Gene Description:
protease, serine 28
Transcript:
RefSeq NM_053259.2 (CURRENT)
Match location:
Position 300 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000428749)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCTAAGAATGTACAGTTAC
Hairpin Sequence:
5'-CCGG-AGCCTAAGAATGTACAGTTAC-CTCGAG-GTAACTGTACATTCTTAGGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCTAAGAATGTACAGTTACCTCGAGGTAACTGTACATTCTTAGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCTAAGAATGTACAGTTACCTCGAGGTAACTGTACATTCTTAGGCT-3'

Other clones with same target sequence:

(none)