Construct: shRNA TRCN0000429202

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011073.3-347s21c1
DNA Barcode:
AGCTCACACTGCCAGCGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prf1 (18646)
Gene Description:
perforin 1 (pore forming protein)
Transcript:
RefSeq NM_011073.3 (CURRENT)
Match location:
Position 347 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429202)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTCACACTGCCAGCGTAAT
Hairpin Sequence:
5'-CCGG-AGCTCACACTGCCAGCGTAAT-CTCGAG-ATTACGCTGGCAGTGTGAGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTCACACTGCCAGCGTAATCTCGAGATTACGCTGGCAGTGTGAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTCACACTGCCAGCGTAATCTCGAGATTACGCTGGCAGTGTGAGCT-3'

Other clones with same target sequence:

(none)