Construct: shRNA TRCN0000429392

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029814.1-1061s21c1
DNA Barcode:
GCTGATACATTGATAAGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chmp5 (76959)
Gene Description:
charged multivesicular body protein 5
Transcript:
RefSeq NM_029814.1 (CURRENT)
Match location:
Position 1061 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429392)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATACATTGATAAGATAA
Hairpin Sequence:
5'-CCGG-GCTGATACATTGATAAGATAA-CTCGAG-TTATCTTATCAATGTATCAGC-TTTTTGGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATACATTGATAAGATAACTCGAGTTATCTTATCAATGTATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATACATTGATAAGATAACTCGAGTTATCTTATCAATGTATCAGC-3'

Other clones with same target sequence:

(none)