Construct: shRNA TRCN0000429525

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175374.3-1263s21c1
DNA Barcode:
TTATGAGGCTCTAGTAGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtrf1l (108853)
Gene Description:
mitochondrial translational release factor 1-like
Transcript:
RefSeq NM_175374.3 (CURRENT)
Match location:
Position 1263 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429525)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTATGAGGCTCTAGTAGAAAT
Hairpin Sequence:
5'-CCGG-TTATGAGGCTCTAGTAGAAAT-CTCGAG-ATTTCTACTAGAGCCTCATAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTATGAGGCTCTAGTAGAAATCTCGAGATTTCTACTAGAGCCTCATAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTATGAGGCTCTAGTAGAAATCTCGAGATTTCTACTAGAGCCTCATAA-3'

Other clones with same target sequence:

(none)