Construct: shRNA TRCN0000429551

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153140.2-1398s21c1
DNA Barcode:
ATCAGCTCTGTCTCCCGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab11fip3 (215445)
Gene Description:
RAB11 family interacting protein 3 (class II)
Transcript:
RefSeq NM_153140.2 (CURRENT)
Match location:
Position 1398 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000429551)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCAGCTCTGTCTCCCGAGAT
Hairpin Sequence:
5'-CCGG-ATCAGCTCTGTCTCCCGAGAT-CTCGAG-ATCTCGGGAGACAGAGCTGAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCAGCTCTGTCTCCCGAGATCTCGAGATCTCGGGAGACAGAGCTGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCAGCTCTGTCTCCCGAGATCTCGAGATCTCGGGAGACAGAGCTGAT-3'

Other clones with same target sequence:

(none)