Construct: shRNA TRCN0000430602

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002261.2-835s21c1
DNA Barcode:
GAGTGCTCATGTACCATTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KLRC3 (3823)
Gene Description:
killer cell lectin like receptor C3
Transcript:
RefSeq NM_002261.2 (CURRENT)
Match location:
Position 835 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430602)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGCTCATGTACCATTTAG
Hairpin Sequence:
5'-CCGG-GAGTGCTCATGTACCATTTAG-CTCGAG-CTAAATGGTACATGAGCACTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGCTCATGTACCATTTAGCTCGAGCTAAATGGTACATGAGCACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGCTCATGTACCATTTAGCTCGAGCTAAATGGTACATGAGCACTC-3'

Other clones with same target sequence:

(none)