Construct: shRNA TRCN0000430660

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000270.3-512s21c1
DNA Barcode:
CAAGTTTGAGGTTGGAGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PNP (4860)
Gene Description:
purine nucleoside phosphorylase
Transcript:
RefSeq NM_000270.3 (CURRENT)
Match location:
Position 512 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430660)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGTTTGAGGTTGGAGATAT
Hairpin Sequence:
5'-CCGG-CAAGTTTGAGGTTGGAGATAT-CTCGAG-ATATCTCCAACCTCAAACTTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGTTTGAGGTTGGAGATATCTCGAGATATCTCCAACCTCAAACTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGTTTGAGGTTGGAGATATCTCGAGATATCTCCAACCTCAAACTTG-3'

Other clones with same target sequence:

(none)