Construct: shRNA TRCN0000430850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173771.4-377s21c1
DNA Barcode:
TATCGCAGCAGCACATGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mgat4f (240755)
Gene Description:
MGAT4 family, member F
Transcript:
RefSeq NM_173771.4 (CURRENT)
Match location:
Position 377 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATCGCAGCAGCACATGTAAA
Hairpin Sequence:
5'-CCGG-TATCGCAGCAGCACATGTAAA-CTCGAG-TTTACATGTGCTGCTGCGATA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATCGCAGCAGCACATGTAAACTCGAGTTTACATGTGCTGCTGCGATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATCGCAGCAGCACATGTAAACTCGAGTTTACATGTGCTGCTGCGATA-3'

Other clones with same target sequence:

(none)