Construct: shRNA TRCN0000430918

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173027.2-1159s21c1
DNA Barcode:
GCTCCGTCCTCATAATCTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ip6k3 (271424)
Gene Description:
inositol hexaphosphate kinase 3
Transcript:
RefSeq NM_173027.2 (CURRENT)
Match location:
Position 1159 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430918)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCGTCCTCATAATCTATG
Hairpin Sequence:
5'-CCGG-GCTCCGTCCTCATAATCTATG-CTCGAG-CATAGATTATGAGGACGGAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCGTCCTCATAATCTATGCTCGAGCATAGATTATGAGGACGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCGTCCTCATAATCTATGCTCGAGCATAGATTATGAGGACGGAGC-3'

Other clones with same target sequence:

(none)