Construct: shRNA TRCN0000430947

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172399.3-1006s21c1
DNA Barcode:
CCATACCCTGAATTACCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndnf (68169)
Gene Description:
neuron-derived neurotrophic factor
Transcript:
RefSeq NM_172399.3 (CURRENT)
Match location:
Position 1006 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000430947)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATACCCTGAATTACCATAT
Hairpin Sequence:
5'-CCGG-CCATACCCTGAATTACCATAT-CTCGAG-ATATGGTAATTCAGGGTATGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATACCCTGAATTACCATATCTCGAGATATGGTAATTCAGGGTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATACCCTGAATTACCATATCTCGAGATATGGTAATTCAGGGTATGG-3'

Other clones with same target sequence:

(none)