Construct: shRNA TRCN0000431506

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014555.3-2598s21c1
DNA Barcode:
CATCGTGGTAGAGCGCATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRPM5 (29850)
Gene Description:
transient receptor potential cation channel subfamily M member 5
Transcript:
RefSeq NM_014555.3 (CURRENT)
Match location:
Position 2598 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431506)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGTGGTAGAGCGCATGAT
Hairpin Sequence:
5'-CCGG-CATCGTGGTAGAGCGCATGAT-CTCGAG-ATCATGCGCTCTACCACGATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGTGGTAGAGCGCATGATCTCGAGATCATGCGCTCTACCACGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGTGGTAGAGCGCATGATCTCGAGATCATGCGCTCTACCACGATG-3'

Other clones with same target sequence:

(none)