Construct: shRNA TRCN0000431871

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172238.3-613s21c1
DNA Barcode:
CCAATTCCACTGTCGCCTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TFAP2D (83741)
Gene Description:
transcription factor AP-2 delta
Transcript:
RefSeq NM_172238.3 (CURRENT)
Match location:
Position 613 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000431871)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAATTCCACTGTCGCCTATT
Hairpin Sequence:
5'-CCGG-CCAATTCCACTGTCGCCTATT-CTCGAG-AATAGGCGACAGTGGAATTGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAATTCCACTGTCGCCTATTCTCGAGAATAGGCGACAGTGGAATTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAATTCCACTGTCGCCTATTCTCGAGAATAGGCGACAGTGGAATTGG-3'

Other clones with same target sequence:

(none)