Construct: shRNA TRCN0000432316

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025831.3-1421s21c1
DNA Barcode:
ATGAGCCGGAAGGAGCGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pxdc1 (66895)
Gene Description:
PX domain containing 1
Transcript:
RefSeq NM_025831.3 (CURRENT)
Match location:
Position 1421 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432316)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGAGCCGGAAGGAGCGTTTA
Hairpin Sequence:
5'-CCGG-ATGAGCCGGAAGGAGCGTTTA-CTCGAG-TAAACGCTCCTTCCGGCTCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGAGCCGGAAGGAGCGTTTACTCGAGTAAACGCTCCTTCCGGCTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGAGCCGGAAGGAGCGTTTACTCGAGTAAACGCTCCTTCCGGCTCAT-3'

Other clones with same target sequence:

(none)