Construct: shRNA TRCN0000432402

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001166030.1-327s21c1
DNA Barcode:
ATTTGATCATCGGATGGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mylk4 (238564)
Gene Description:
myosin light chain kinase family, member 4
Transcript:
RefSeq NM_001166030.1 (CURRENT)
Match location:
Position 327 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432402)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTTGATCATCGGATGGTAAT
Hairpin Sequence:
5'-CCGG-ATTTGATCATCGGATGGTAAT-CTCGAG-ATTACCATCCGATGATCAAAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTTGATCATCGGATGGTAATCTCGAGATTACCATCCGATGATCAAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTTGATCATCGGATGGTAATCTCGAGATTACCATCCGATGATCAAAT-3'

Other clones with same target sequence:

(none)