Construct: shRNA TRCN0000432724

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001166030.1-1227s21c1
DNA Barcode:
ATCCATGAAAGGAGCCTATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mylk4 (238564)
Gene Description:
myosin light chain kinase family, member 4
Transcript:
RefSeq NM_001166030.1 (CURRENT)
Match location:
Position 1227 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432724)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCATGAAAGGAGCCTATTC
Hairpin Sequence:
5'-CCGG-ATCCATGAAAGGAGCCTATTC-CTCGAG-GAATAGGCTCCTTTCATGGAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCATGAAAGGAGCCTATTCCTCGAGGAATAGGCTCCTTTCATGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCATGAAAGGAGCCTATTCCTCGAGGAATAGGCTCCTTTCATGGAT-3'

Other clones with same target sequence:

(none)