Construct: shRNA TRCN0000433025

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005320.2-176s21c1
DNA Barcode:
GTATCTGAGCTTATCACCAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H1-3 (3007)
Gene Description:
H1.3 linker histone, cluster member
Transcript:
RefSeq NM_005320.2 (CURRENT)
Match location:
Position 176 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433025)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATCTGAGCTTATCACCAAG
Hairpin Sequence:
5'-CCGG-GTATCTGAGCTTATCACCAAG-CTCGAG-CTTGGTGATAAGCTCAGATAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATCTGAGCTTATCACCAAGCTCGAGCTTGGTGATAAGCTCAGATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATCTGAGCTTATCACCAAGCTCGAGCTTGGTGATAAGCTCAGATAC-3'

Other clones with same target sequence:

(none)