Construct: shRNA TRCN0000433296

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009544.2-1514s21c1
DNA Barcode:
AGTCAGCTATCTTGCCTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp105 (22646)
Gene Description:
zinc finger protein 105
Transcript:
RefSeq NM_009544.2 (CURRENT)
Match location:
Position 1514 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433296)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCAGCTATCTTGCCTTATT
Hairpin Sequence:
5'-CCGG-AGTCAGCTATCTTGCCTTATT-CTCGAG-AATAAGGCAAGATAGCTGACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCAGCTATCTTGCCTTATTCTCGAGAATAAGGCAAGATAGCTGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCAGCTATCTTGCCTTATTCTCGAGAATAAGGCAAGATAGCTGACT-3'

Other clones with same target sequence:

(none)