Construct: shRNA TRCN0000433302

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173182.2-3816s21c1
DNA Barcode:
TTTACGCTGTTTCGTTCTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fndc3b (72007)
Gene Description:
fibronectin type III domain containing 3B
Transcript:
RefSeq NM_173182.2 (CURRENT)
Match location:
Position 3816 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433302)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTACGCTGTTTCGTTCTTTG
Hairpin Sequence:
5'-CCGG-TTTACGCTGTTTCGTTCTTTG-CTCGAG-CAAAGAACGAAACAGCGTAAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTACGCTGTTTCGTTCTTTGCTCGAGCAAAGAACGAAACAGCGTAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTACGCTGTTTCGTTCTTTGCTCGAGCAAAGAACGAAACAGCGTAAA-3'

Other clones with same target sequence:

(none)