Construct: shRNA TRCN0000433409

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026222.2-1500s21c1
DNA Barcode:
GAACGCAGAATGTCACGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc39 (51938)
Gene Description:
coiled-coil domain containing 39
Transcript:
RefSeq NM_026222.2 (CURRENT)
Match location:
Position 1500 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433409)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACGCAGAATGTCACGATTA
Hairpin Sequence:
5'-CCGG-GAACGCAGAATGTCACGATTA-CTCGAG-TAATCGTGACATTCTGCGTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACGCAGAATGTCACGATTACTCGAGTAATCGTGACATTCTGCGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACGCAGAATGTCACGATTACTCGAGTAATCGTGACATTCTGCGTTC-3'

Other clones with same target sequence:

(none)