Construct: shRNA TRCN0000433527

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003545.3-30s21c1
DNA Barcode:
ACTGGGTAAAGGAGGCGCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H4C5 (8367)
Gene Description:
H4 clustered histone 5
Transcript:
RefSeq NM_003545.3 (CURRENT)
Match location:
Position 30 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433527)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGGGTAAAGGAGGCGCTAA
Hairpin Sequence:
5'-CCGC-ACTGGGTAAAGGAGGCGCTAA-CTCGAG-TTAGCGCCTCCTTTACCCAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGGGTAAAGGAGGCGCTAACTCGAGTTAGCGCCTCCTTTACCCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGGGTAAAGGAGGCGCTAACTCGAGTTAGCGCCTCCTTTACCCAGT-3'

Other clones with same target sequence:

(none)