Transcript: Human NM_003545.3

Homo sapiens H4 clustered histone 5 (H4C5), mRNA.

Source:
NCBI, updated 2019-07-25
Taxon:
Homo sapiens (human)
Gene:
H4C5 (8367)
Length:
377
CDS:
1..312

Additional Resources:

NCBI RefSeq record:
NM_003545.3
NBCI Gene record:
H4C5 (8367)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_003545.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000106993 TAAGGTCCTGCGAGATAACAT pLKO.1 60 CDS 100% 5.625 7.875 N H4C5 n/a
2 TRCN0000106992 GATTCGTGATGCTGTGACTTA pLKO.1 198 CDS 100% 4.950 3.465 N H4C5 n/a
3 TRCN0000433527 ACTGGGTAAAGGAGGCGCTAA pLKO_005 30 CDS 100% 4.050 2.835 N H4C5 n/a
4 TRCN0000428242 GACAGTGACAGCGATGGATGT pLKO_005 240 CDS 100% 4.050 2.835 N H4C5 n/a
5 TRCN0000106991 CGTCACCGTAAGGTCCTGCGA pLKO.1 52 CDS 100% 0.000 0.000 N H4C5 n/a
6 TRCN0000106990 CGAGATAACATCCAGGGCATT pLKO.1 70 CDS 100% 4.050 2.430 N H4C5 n/a
7 TRCN0000106994 GTTCTGAAGGTGTTTCTGGAA pLKO.1 172 CDS 100% 2.640 1.584 N H4C5 n/a
8 TRCN0000425361 AGCGCATTTCTGGTCTCATCT pLKO_005 134 CDS 100% 4.950 2.475 Y H4C5 n/a
9 TRCN0000433202 ATGTGGTCTACGCGCTGAAGA pLKO_005 257 CDS 100% 4.950 2.475 Y H4C5 n/a
10 TRCN0000074267 GCGCATTTCTGGTCTCATCTA pLKO.1 135 CDS 100% 4.950 2.475 Y H4-16 n/a
11 TRCN0000106959 GATAACATCCAGGGCATTACA pLKO.1 73 CDS 100% 5.625 2.813 Y H4C3 n/a
12 TRCN0000300504 GATAACATCCAGGGCATTACA pLKO_005 73 CDS 100% 5.625 2.813 Y H4C3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_003545.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01915 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01915 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000475279 TATAGCGGGACGTGTAGAGTAGCT pLX_317 27.6% 100% 100% V5 n/a
4 ccsbBroadEn_11268 pDONR223 100% 89.6% 100% None (many diffs) n/a
5 ccsbBroad304_11268 pLX_304 0% 89.6% 100% V5 (many diffs) n/a
6 TRCN0000473699 AAATTGTTGTTGTCACATGCCGTA pLX_317 100% 89.6% 100% V5 (many diffs) n/a
7 ccsbBroadEn_07227 pDONR223 100% 88.6% 99% None (many diffs) n/a
8 ccsbBroad304_07227 pLX_304 0% 88.6% 99% V5 (many diffs) n/a
9 TRCN0000466667 ACAGCTATGCTGGGTCCGATAGTT pLX_317 69.3% 88.6% 99% V5 (many diffs) n/a
10 ccsbBroadEn_01912 pDONR223 100% 85.4% 100% None (many diffs) n/a
11 ccsbBroad304_01912 pLX_304 0% 85.4% 100% V5 (many diffs) n/a
12 TRCN0000472437 ACTCCACAACGATCTTTCCTTCAT pLX_317 100% 85.4% 100% V5 (many diffs) n/a
13 ccsbBroadEn_07216 pDONR223 100% 84.7% 100% None (many diffs) n/a
14 ccsbBroad304_07216 pLX_304 0% 84.7% 100% V5 (many diffs) n/a
15 TRCN0000468808 CGAAACCTGTGAAAGAAATAGCCA pLX_317 100% 84.7% 100% V5 (many diffs) n/a
16 ccsbBroadEn_01883 pDONR223 100% 84.4% 100% None (many diffs) n/a
17 ccsbBroad304_01883 pLX_304 0% 84.4% 100% V5 (many diffs) n/a
18 TRCN0000470786 AAACACGGTAGAGCCAAGTCGACG pLX_317 100% 84.4% 100% V5 (many diffs) n/a
19 ccsbBroadEn_01914 pDONR223 100% 84.1% 100% None (many diffs) n/a
20 ccsbBroad304_01914 pLX_304 0% 84.1% 100% V5 (many diffs) n/a
21 TRCN0000470478 ACCAGTGCAGTTCTATCGAGTATT pLX_317 100% 84.1% 100% V5 (many diffs) n/a
22 ccsbBroadEn_01910 pDONR223 100% 83.8% 100% None (many diffs) n/a
23 ccsbBroad304_01910 pLX_304 0% 83.8% 100% V5 (many diffs) n/a
24 TRCN0000470331 GGAGTACAACATTTCGGGTCCGAA pLX_317 100% 83.8% 100% V5 (many diffs) n/a
25 ccsbBroadEn_01916 pDONR223 100% 83.4% 100% None (many diffs) n/a
26 ccsbBroad304_01916 pLX_304 0% 83.4% 100% V5 (many diffs) n/a
27 TRCN0000468855 AAAAGGGCACCCCGTTGTGATCCC pLX_317 100% 83.4% 100% V5 (many diffs) n/a
28 ccsbBroadEn_05703 pDONR223 100% 82.2% 100% None (many diffs) n/a
29 ccsbBroad304_05703 pLX_304 0% 82.2% 100% V5 (many diffs) n/a
30 TRCN0000478553 TTTATTGTGCGATTTATCCGATTA pLX_317 82.9% 82.2% 100% V5 (many diffs) n/a
31 ccsbBroadEn_01913 pDONR223 100% 81.2% 100% None (many diffs) n/a
32 ccsbBroad304_01913 pLX_304 0% 81.2% 100% V5 (many diffs) n/a
33 TRCN0000465747 GTATTTCCGCGAATCGGGTCGGAG pLX_317 100% 81.2% 100% V5 (many diffs) n/a
34 ccsbBroadEn_01911 pDONR223 100% 80.9% 100% None (many diffs) n/a
35 ccsbBroad304_01911 pLX_304 0% 80.9% 100% V5 (many diffs) n/a
36 TRCN0000472587 AGGCTCCAGTCGACTTCTCCCACC pLX_317 100% 80.9% 100% V5 (many diffs) n/a
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