Construct: shRNA TRCN0000433779

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019967.2-2912s21c1
DNA Barcode:
ACGTGTTCTAGCGACTGTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Brinp1 (56710)
Gene Description:
bone morphogenic protein/retinoic acid inducible neural specific 1
Transcript:
RefSeq NM_019967.2 (CURRENT)
Match location:
Position 2912 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433779)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTGTTCTAGCGACTGTATA
Hairpin Sequence:
5'-CCGG-ACGTGTTCTAGCGACTGTATA-CTCGAG-TATACAGTCGCTAGAACACGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTGTTCTAGCGACTGTATACTCGAGTATACAGTCGCTAGAACACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTGTTCTAGCGACTGTATACTCGAGTATACAGTCGCTAGAACACGT-3'

Other clones with same target sequence:

(none)