Construct: shRNA TRCN0000434244

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025285.2-706s21c1
DNA Barcode:
GAAGTGTATGACATGGTTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stmn2 (20257)
Gene Description:
stathmin-like 2
Transcript:
RefSeq NM_025285.2 (CURRENT)
Match location:
Position 706 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434244)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTGTATGACATGGTTTAA
Hairpin Sequence:
5'-CCGG-GAAGTGTATGACATGGTTTAA-CTCGAG-TTAAACCATGTCATACACTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTGTATGACATGGTTTAACTCGAGTTAAACCATGTCATACACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTGTATGACATGGTTTAACTCGAGTTAAACCATGTCATACACTTC-3'

Other clones with same target sequence:

(none)