Construct: shRNA TRCN0000434979

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138691.2-1958s21c1
DNA Barcode:
AGCTAGTAAAGGCCAATATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMC1 (117531)
Gene Description:
transmembrane channel like 1
Transcript:
RefSeq NM_138691.2 (CURRENT)
Match location:
Position 1958 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434979)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTAGTAAAGGCCAATATTA
Hairpin Sequence:
5'-CCGG-AGCTAGTAAAGGCCAATATTA-CTCGAG-TAATATTGGCCTTTACTAGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTAGTAAAGGCCAATATTACTCGAGTAATATTGGCCTTTACTAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTAGTAAAGGCCAATATTACTCGAGTAATATTGGCCTTTACTAGCT-3'

Other clones with same target sequence:

(none)