Construct: shRNA TRCN0000435013

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007525.3-1687s21c1
DNA Barcode:
AGGAATGCTGTTAACATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bard1 (12021)
Gene Description:
BRCA1 associated RING domain 1
Transcript:
RefSeq NM_007525.3 (CURRENT)
Match location:
Position 1687 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435013)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAATGCTGTTAACATATTT
Hairpin Sequence:
5'-CCGG-AGGAATGCTGTTAACATATTT-CTCGAG-AAATATGTTAACAGCATTCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAATGCTGTTAACATATTTCTCGAGAAATATGTTAACAGCATTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAATGCTGTTAACATATTTCTCGAGAAATATGTTAACAGCATTCCT-3'

Other clones with same target sequence:

(none)