Construct: shRNA TRCN0000435422

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133898.4-1250s21c1
DNA Barcode:
GAGACCTGAGCCGTCACTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
N4bp2l1 (100637)
Gene Description:
NEDD4 binding protein 2-like 1
Transcript:
RefSeq NM_133898.4 (CURRENT)
Match location:
Position 1250 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435422)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACCTGAGCCGTCACTTTA
Hairpin Sequence:
5'-CCGG-GAGACCTGAGCCGTCACTTTA-CTCGAG-TAAAGTGACGGCTCAGGTCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACCTGAGCCGTCACTTTACTCGAGTAAAGTGACGGCTCAGGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACCTGAGCCGTCACTTTACTCGAGTAAAGTGACGGCTCAGGTCTC-3'

Other clones with same target sequence:

(none)