Construct: shRNA TRCN0000436203

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010137.3-1426s21c1
DNA Barcode:
GTATCATGTGTGTCAACTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Epas1 (13819)
Gene Description:
endothelial PAS domain protein 1
Transcript:
RefSeq NM_010137.3 (CURRENT)
Match location:
Position 1426 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436203)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATCATGTGTGTCAACTATG
Hairpin Sequence:
5'-CCGG-GTATCATGTGTGTCAACTATG-CTCGAG-CATAGTTGACACACATGATAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATCATGTGTGTCAACTATGCTCGAGCATAGTTGACACACATGATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATCATGTGTGTCAACTATGCTCGAGCATAGTTGACACACATGATAC-3'

Other clones with same target sequence:

(none)