Construct: shRNA TRCN0000436247

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010581.3-895s21c1
DNA Barcode:
CTCTTTCACCATTGCCATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cd47 (16423)
Gene Description:
CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
Transcript:
RefSeq NM_010581.3 (CURRENT)
Match location:
Position 895 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436247)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTTTCACCATTGCCATATT
Hairpin Sequence:
5'-CCGG-CTCTTTCACCATTGCCATATT-CTCGAG-AATATGGCAATGGTGAAAGAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTTTCACCATTGCCATATTCTCGAGAATATGGCAATGGTGAAAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTTTCACCATTGCCATATTCTCGAGAATATGGCAATGGTGAAAGAG-3'

Other clones with same target sequence:

(none)