Construct: shRNA TRCN0000436311
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_008598.2-704s21c1
- DNA Barcode:
- TGAGCCGTCTGGCCGAAATTG
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Mgmt (17314)
- Gene Description:
- O-6-methylguanine-DNA methyltransferase
- Transcript:
- RefSeq NM_008598.2 (CURRENT)
- Match location:
- Position 704 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000436311)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TGAGCCGTCTGGCCGAAATTG
- Hairpin Sequence:
-
5'-CCGG-TGAGCCGTCTGGCCGAAATTG-CTCGAG-CAATTTCGGCCAGACGGCTCA-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTGAGCCGTCTGGCCGAAATTGCTCGAGCAATTTCGGCCAGACGGCTCATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATGAGCCGTCTGGCCGAAATTGCTCGAGCAATTTCGGCCAGACGGCTCA-3'
Other clones with same target sequence:
(none)
