Construct: shRNA TRCN0000436341

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025963.3-85s21c1
DNA Barcode:
TTTAAGGAGGGCGTGATGGTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rps10 (67097)
Gene Description:
ribosomal protein S10
Transcript:
RefSeq NM_025963.3 (CURRENT)
Match location:
Position 85 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436341)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTAAGGAGGGCGTGATGGTC
Hairpin Sequence:
5'-CCGG-TTTAAGGAGGGCGTGATGGTC-CTCGAG-GACCATCACGCCCTCCTTAAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTAAGGAGGGCGTGATGGTCCTCGAGGACCATCACGCCCTCCTTAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTAAGGAGGGCGTGATGGTCCTCGAGGACCATCACGCCCTCCTTAAA-3'

Other clones with same target sequence:

(none)