Construct: shRNA TRCN0000436563

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010469.2-2235s21c1
DNA Barcode:
TAAACCCTTGGCGAGTCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hoxd4 (15436)
Gene Description:
homeobox D4
Transcript:
RefSeq NM_010469.2 (CURRENT)
Match location:
Position 2235 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436563)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAACCCTTGGCGAGTCATTA
Hairpin Sequence:
5'-CCGG-TAAACCCTTGGCGAGTCATTA-CTCGAG-TAATGACTCGCCAAGGGTTTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAACCCTTGGCGAGTCATTACTCGAGTAATGACTCGCCAAGGGTTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAACCCTTGGCGAGTCATTACTCGAGTAATGACTCGCCAAGGGTTTA-3'

Other clones with same target sequence:

(none)