Construct: shRNA TRCN0000437003

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005020.1-934s21c1
DNA Barcode:
TGACGGAGCTGGAGATCTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDE1C (5137)
Gene Description:
phosphodiesterase 1C
Transcript:
RefSeq NM_005020.1 (NON-CURRENT)
Match location:
Position 934 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437003)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACGGAGCTGGAGATCTTTG
Hairpin Sequence:
5'-CCGG-TGACGGAGCTGGAGATCTTTG-CTCGAG-CAAAGATCTCCAGCTCCGTCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACGGAGCTGGAGATCTTTGCTCGAGCAAAGATCTCCAGCTCCGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACGGAGCTGGAGATCTTTGCTCGAGCAAAGATCTCCAGCTCCGTCA-3'

Other clones with same target sequence:

(none)