Construct: shRNA TRCN0000437066

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027310.3-664s21c1
DNA Barcode:
AGTAGAACCCAAGGTCCATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Meaf6 (70088)
Gene Description:
MYST/Esa1-associated factor 6
Transcript:
RefSeq NM_027310.3 (NON-CURRENT)
Match location:
Position 664 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437066)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTAGAACCCAAGGTCCATTG
Hairpin Sequence:
5'-CCGG-AGTAGAACCCAAGGTCCATTG-CTCGAG-CAATGGACCTTGGGTTCTACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTAGAACCCAAGGTCCATTGCTCGAGCAATGGACCTTGGGTTCTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTAGAACCCAAGGTCCATTGCTCGAGCAATGGACCTTGGGTTCTACT-3'

Other clones with same target sequence:

(none)