Construct: shRNA TRCN0000437157

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178655.3-726s21c1
DNA Barcode:
CATTATCCCACCTCGGAAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ank2 (109676)
Gene Description:
ankyrin 2, brain
Transcript:
RefSeq NM_178655.3 (CURRENT)
Match location:
Position 726 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437157)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTATCCCACCTCGGAAATG
Hairpin Sequence:
5'-CCGG-CATTATCCCACCTCGGAAATG-CTCGAG-CATTTCCGAGGTGGGATAATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTATCCCACCTCGGAAATGCTCGAGCATTTCCGAGGTGGGATAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTATCCCACCTCGGAAATGCTCGAGCATTTCCGAGGTGGGATAATG-3'

Other clones with same target sequence:

(none)