Construct: shRNA TRCN0000437219

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008110.2-1598s21c1
DNA Barcode:
TGCAGTGTCCGTAGGTGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gdf9 (14566)
Gene Description:
growth differentiation factor 9
Transcript:
RefSeq NM_008110.2 (CURRENT)
Match location:
Position 1598 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437219)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCAGTGTCCGTAGGTGTAAA
Hairpin Sequence:
5'-CCGG-TGCAGTGTCCGTAGGTGTAAA-CTCGAG-TTTACACCTACGGACACTGCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCAGTGTCCGTAGGTGTAAACTCGAGTTTACACCTACGGACACTGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCAGTGTCCGTAGGTGTAAACTCGAGTTTACACCTACGGACACTGCA-3'

Other clones with same target sequence:

(none)