Construct: shRNA TRCN0000437719

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198958.2-1131s21c1
DNA Barcode:
AGACTGGACAGAGGCGTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nox3 (224480)
Gene Description:
NADPH oxidase 3
Transcript:
RefSeq NM_198958.2 (CURRENT)
Match location:
Position 1131 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437719)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACTGGACAGAGGCGTTATT
Hairpin Sequence:
5'-CCGG-AGACTGGACAGAGGCGTTATT-CTCGAG-AATAACGCCTCTGTCCAGTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACTGGACAGAGGCGTTATTCTCGAGAATAACGCCTCTGTCCAGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACTGGACAGAGGCGTTATTCTCGAGAATAACGCCTCTGTCCAGTCT-3'

Other clones with same target sequence:

(none)