Construct: shRNA TRCN0000437979

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172704.3-703s21c1
DNA Barcode:
GGTCTCAAGCTGTTCCGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnajc11 (230935)
Gene Description:
DnaJ heat shock protein family (Hsp40) member C11
Transcript:
RefSeq NM_172704.3 (CURRENT)
Match location:
Position 703 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000437979)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCTCAAGCTGTTCCGTAAT
Hairpin Sequence:
5'-CCGG-GGTCTCAAGCTGTTCCGTAAT-CTCGAG-ATTACGGAACAGCTTGAGACC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCTCAAGCTGTTCCGTAATCTCGAGATTACGGAACAGCTTGAGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCTCAAGCTGTTCCGTAATCTCGAGATTACGGAACAGCTTGAGACC-3'

Other clones with same target sequence:

(none)