Construct: shRNA TRCN0000439146

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005322.2-513s21c1
DNA Barcode:
AGACTCCGAAGAAGGCGAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H1-5 (3009)
Gene Description:
H1.5 linker histone, cluster member
Transcript:
RefSeq NM_005322.2 (CURRENT)
Match location:
Position 513 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000439146)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACTCCGAAGAAGGCGAAGA
Hairpin Sequence:
5'-CCGG-AGACTCCGAAGAAGGCGAAGA-CTCGAG-TCTTCGCCTTCTTCGGAGTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACTCCGAAGAAGGCGAAGACTCGAGTCTTCGCCTTCTTCGGAGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACTCCGAAGAAGGCGAAGACTCGAGTCTTCGCCTTCTTCGGAGTCT-3'

Other clones with same target sequence:

(none)