Construct: shRNA TRCN0000439287
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_025790.2-524s21c1
- DNA Barcode:
- CATTTGCCTCAGTGGATCTGA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Acot13 (66834)
- Gene Description:
- acyl-CoA thioesterase 13
- Transcript:
- RefSeq NM_025790.2 (CURRENT)
- Match location:
- Position 524 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000439287)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CATTTGCCTCAGTGGATCTGA
- Hairpin Sequence:
-
5'-CCGC-CATTTGCCTCAGTGGATCTGA-CTCGAG-TCAGATCCACTGAGGCAAATG-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCATTTGCCTCAGTGGATCTGACTCGAGTCAGATCCACTGAGGCAAATGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACATTTGCCTCAGTGGATCTGACTCGAGTCAGATCCACTGAGGCAAATG-3'
Other clones with same target sequence:
(none)
